FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n01_TR_545-3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n01_TR_545-3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116447
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA9250.072.9072951
GAGCGAA4300.069.142521
GCGAAAG9950.068.50082
CGAAAGC10100.068.201373
GAGCAAA23000.054.5441321
CAAAAGC29800.041.8507354
GCAAAAG30600.040.5196383
AGCAAAA32800.037.5807952
AAAAGCA44850.028.4538545
CTACGCC1306.489004E-427.880514145
AAAGCAG57400.027.0329076
TATACTG8550.022.0494675
GGAGCGA2353.703634E-521.600311
AAGCAGG69000.021.536697
GAAAGCA33450.021.460014
ACTCCGC2155.061657E-420.2295848
CTATACT8100.019.6937564
ATACTGC6001.8189894E-1118.1272076
GTACTAT7400.017.6388631
CTATACA7850.017.5499084
Produced by FastQC (version 0.11.5)