FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n01_TR_540-7_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n01_TR_540-7_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1679176
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC46060.27430120487667764No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA37750.2248126462026613No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA36300.21617745846772463No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC34260.20402864261995168No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG33220.19783512865834194No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA27970.16656979375598507No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG23390.13929451111735755No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA22810.1358404360233829No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT19630.11690257602538388No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG19240.11458000828978024No Hit
CCATCTCAACAGATGCAGAAAAAGCATTTGATAGCGCTGCTACCAAATCA18370.10939889564881822No Hit
ACATTCTACTGAACAACCAATTGGGTTCATGGGCAAACCAAAATAGGAAC17940.10683811583776805No Hit
AAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTTGCT17850.10630213866801336No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG17840.10624258564915173No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6300.0113.987921
ACGGTAT502.2655513E-887.021986
GTTACGT3050.085.6183551
AGCGAAA9150.079.2552
TTACGTG3300.079.110892
CGGTATT554.4012268E-879.077917
CGAAAGC9650.075.148514
TACGTGT3600.072.518323
GCGAAAG10450.072.171353
AATGGGT13100.068.6146857
CGGTCGG554.0502637E-665.943431
ATACCGG350.003563356862.158566
GCGATTA1800.060.406742145
CTATAAC1154.0017767E-1156.7534684
AACGGGT6350.055.959023
AGCTACT5900.055.2875258
ATGGGTG16500.054.4758958
GATACCG400.00604555254.388745
TACCGGA400.00605553354.366077
GTCGTCT1353.6379788E-1253.694878145
Produced by FastQC (version 0.11.5)