FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n01_TR_531-5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n01_TR_531-5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1612831
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG84150.5217533641156451No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA70160.43501147981406607No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA63240.39210555848690903No Hit
CAAACAGCAGACAAGAGAATAATGGACATGATTCCAGAGACACATCAGGA38690.2398887422178765No Hit
GTCTCTGGAATCATGTCCATTATTCTCTTGTCTGCTGTTTGATTATGGCC37780.2342464895578024No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT37160.23040231741577386No Hit
CAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGA35750.22165992593148323No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC33060.2049811790571982No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG31210.19351066540759695No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT30600.18972849604205275No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA28290.1754058546741723No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT27880.17286374083831474No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC27600.17112766309675348No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG25850.16027717721199555No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA24890.1543249106694998No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT24720.15327086346926616No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC22020.1365301138184968No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT21890.1357240777242005No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT21660.13429801386506088No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT21610.13398799998263922No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT21190.13138388337029733No Hit
TCCACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT20880.12946179729928306No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC20790.1289037723109241No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA20730.1285317556520181No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC20330.12605164459264484No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT20270.12567962793373885No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA19970.12381954463920894No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA19950.12369553908624029No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC19710.12220747245061635No Hit
GTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATC19470.12071940581499238No Hit
GTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTAGTGAGTATCT19420.12040939193257075No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT19260.11941734750882144No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC19100.11842530308507214No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC19060.11817729197913483No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC18890.1171232447789012No Hit
TGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTC18550.11501515037843395No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG18490.11464313371952796No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG18270.11327907263687267No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT18070.11203901710718607No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT18020.1117290032247644No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG18000.11160499767179574No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG17990.1115429948953114No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT17260.10701679221195524No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC16900.10478469225851934No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCAGG16770.10397865616422304No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG16450.10199456731672443No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG16440.10193256454024012No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC16220.10056850345758483No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTTA100.007081977145.063551
AGTCGGC100.0070865755145.032065
GAGCGAA5400.0142.37721
ATATAAT259.371469E-487.038141
GAGCAAA68250.083.956211
GCAGGTA19500.082.1568159
AGCGAAA12650.080.8456951
GCAGGGT26600.080.6670159
AAGCAGG84700.076.8561867
GCGAAAG13950.075.375092
GGCGAAA406.64997E-572.531781
GCGTCAG406.64997E-572.531781
ACTAGTT300.001934260672.516042
CGAAAGC14650.071.773553
GACAGTA3250.071.376057
AGCAAAA85050.067.528162
GTAGAAA12900.064.660111
TTATAGG1500.062.8608741
AGCAGGT45050.062.1124158
AGCAGGG42950.061.604958
Produced by FastQC (version 0.11.5)