FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n01_TR_528-3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n01_TR_528-3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1387638
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA8750.085.346951
GAGCAAA43300.067.647591
CAAAAGC50350.057.7331774
AGCGAAA13350.057.568311
GCGAAAG13700.057.145732
GCAAAAG52800.055.0542763
CGAAAGC14650.054.4296653
AGCAAAA57400.050.7685472
AAAGCAG78500.046.8186456
GTTACGT1751.8189894E-1245.5736161
GGCCGTA803.738325E-545.3065072
AAGCAGG92800.038.037737
AAAAGCA78450.036.9613345
TTACGTG2755.456968E-1231.6321812
CGGAGTA2603.7271093E-927.8809284
GTCGTCT1358.0956577E-426.8454145
GCGATTA1400.001001057725.886635145
GCAGGGG41050.025.7794329
CGAAGCG2351.3338486E-624.6749219
TCGGAGT2951.430999E-824.5730233
Produced by FastQC (version 0.11.5)