FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n01_TR_427-5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n01_TR_427-5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1546500
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT34230.2213385063045587No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT26510.17141933397995474No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT25980.16799224054316197No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC25360.16398318784351762No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA24810.16042677012609116No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC24780.16023278370514066No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA24610.15913352731975428No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC20640.13346265761396703No Hit
GAAAGATGACCTTCTTGAAAATTTGCAGGCCTACCAGAAGCGAATGGGAG20340.13152279340446169No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG19910.12874232137083738No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT19870.12848367280957No Hit
GATTCGCTTGGAGAAACTGTGATGAGAATGGGAGACCTTCACTACCTCCA18440.11923698674426123No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT17630.11399935337859683No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT17120.11070158422243777No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT16970.1097316521176851No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCAGG16420.10617523440025864No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG16070.1039120594891691No Hit
GAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG15760.1019075331393469No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA10700.092.857351
GAGCGAA3800.085.883241
CGAAAGC12350.081.012353
GCGAAAG12750.078.470782
CTGTACG553.2220854E-452.7188769
AGGTCGT2300.047.2827266
GGTCGTT2450.044.380697
TCTACAC8600.040.4652183
CTACACT10500.036.595334
ACGTTGT800.00203855136.244239
CAATTCG3200.033.9789669
GAAAGCA31650.032.7567944
GCACCGT4200.031.0715056
ATCCCGA4750.030.5263926
GTTACGT950.004728030430.526396
TATACGT1204.0590035E-430.2084064
GTAGAAA19900.029.884111
GGATAGC4450.029.3259125
AGCACCG4950.029.2930035
ACCGTCT3850.028.2422568
Produced by FastQC (version 0.11.5)