FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n01_TR_286-5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n01_TR_286-5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1211833
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC26300.21702660350064734No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG19810.16347136940486023No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA19510.160995780771773No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA18300.15101090661832117No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC16700.13780776724185592No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA16380.13516713936656288No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA13900.11470227333304175No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12790.105542595390619No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA5700.080.149691
GTTACGT2700.075.202181
TACGTGT3100.065.493263
TTACGTG3150.064.453692
TACCGTC350.003564658862.151772
CGAAAGC8150.058.7200783
AGCGAAA8300.057.6636351
GCGAAAG8800.055.2067833
GAGCAAA40050.053.957261
ACTCCGA3400.051.188041
GTTATGT8000.050.7614671
AATGGGT10550.047.4101687
TCCGAGG3550.046.978573
ACGTGTT4450.045.6245234
GTCGTCT1351.0275471E-842.956715145
CAAAAGC48750.042.688174
CGGGTTG2750.042.187875
AGACGAC2600.041.832923
ATGGGTG12000.041.6814428
GGTTATA3350.041.1286961
Produced by FastQC (version 0.11.5)