FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n01_TR_283-5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n01_TR_283-5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1400630
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG38230.27294860170066326No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA24750.1767061964972905No Hit
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC22340.1594996537272513No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG20770.1482904121716656No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC19150.13672418840093387No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA18550.13244040181918137No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA17510.1250151717441437No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA17250.12315886422538429No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA15640.11166403689768176No Hit
CCCTGTGCCATCCCCCACTGCCACCCTGCTGCACCCCACACACCCACCGC15500.11066448669527285No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGA100.007093846144.98166145
GAGCGAA5600.0113.979261
GTTACGT3350.082.275391
TTACGTG3400.081.048092
TACGTGT3450.079.873483
AGCGAAA8450.075.520362
GCGAAAG9300.070.9572143
CGAAAGC9200.070.152044
CTATAAC753.756304E-758.0133744
AGCTACT3700.056.8171358
GAGCAAA45200.056.324831
GCAGGTA8550.055.1099859
TAGTCGG2200.052.72068
GTTATGT7350.052.302171
CGTTCTG701.6940246E-551.7976536
CGTGTTG5400.051.0302775
AGTCGGC1004.6966306E-850.7616965
ATGTCGA604.9349276E-448.354841
CGGTTCT1056.896698E-848.3444755
GATATAC3500.047.653843
Produced by FastQC (version 0.11.5)