FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n01_TR_199-7_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n01_TR_199-7_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1077984
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA19620.1820064119690088No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA16830.15612476623029656No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG15610.14480734407931845No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA14710.13645842609908868No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC13880.12875886840621012No Hit
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC13310.12347122035206459No Hit
CCATCTCAACAGATGCAGAAAAAGCATTTGATAGCGCTGCTACCAAATCA11390.10566019532757442No Hit
CCCTGTGCCATCCCCCACTGCCACCCTGCTGCACCCCACACACCCACCGC11300.10482530352955147No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG10890.10102190756078012No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATT203.8684037E-4108.733997
GAGCGAA2550.0108.0634161
ACGGTAT259.3872467E-486.9993066
TACGTGT2350.086.3822863
TTACGTG2400.084.582662
GTTACGT2550.079.625671
AGTCGGC1900.076.3151865
CAGTCGG2550.059.7054064
ACTCCGA3200.058.919441
AGCGAAA4700.058.6165542
CGAAAGC4900.056.2240454
GTCGGCT2600.055.7687876
GAAGGCG1450.054.9995574
TCCGAGG3450.054.637243
ACGTGTT4150.052.4092184
GCGAAAG6150.050.690653
CGGCTGG3150.050.6274728
ATGTCGA1056.895061E-848.3441621
AGACGAC2150.047.2089273
GAGCAAA25800.046.3766671
Produced by FastQC (version 0.11.5)