FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n01_TR_197-7_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n01_TR_197-7_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1482740
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG30830.20792586697600388No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA21530.1452041490753605No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA20520.13839243562593576No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG19740.13313190444717213No Hit
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC18720.12625274829032737No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC16890.11391073283245884No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA16550.11161768078017725No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA3850.0126.207521
TATTCGC301.5972813E-596.696311
AGTCGGC1400.072.507555
AGCGAAA6900.070.405892
GTTACGT2650.065.680521
GTAACTC2000.065.2568053
TACGTGT2700.064.4511643
CGAAAGC7550.064.344454
TTACGTG2750.063.279322
GCGAAAG8250.062.400443
AGCTACT3050.054.664928
GGACCGT400.006044124354.3916741
GGTTATA2200.052.7434431
AGTCCGC2350.052.43989145
CAGTCGG2000.050.755294
GGTCGTT1004.710273E-850.7433137
GCAGGTA10050.050.4908529
TATGTGT8500.048.6227153
CTCGCAA604.9433176E-448.338373
GAGCAAA45150.047.223781
Produced by FastQC (version 0.11.5)