FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n01_TR_156-7_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n01_TR_156-7_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1785590
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG33910.1899092176815506No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC28780.1611792180735779No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA27160.15210658661842866No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA25300.14168986161436836No Hit
CCCTGTGCCATCCCCCACTGCCACCCTGCTGCACCCCACACACCCACCGC24830.13905767841441763No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA24220.13564144064426883No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG24100.13496939386981335No Hit
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC22720.12724085596357507No Hit
CCATCTCAACAGATGCAGAAAAAGCATTTGATAGCGCTGCTACCAAATCA22650.12684882867847602No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT20080.11245582692555403No Hit
AGTCAGTGGAGCTCTGTGACTCCTGATCTTGTGGTTTTGAGTTTCAACCC19730.1104956905000588No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA18350.10276715259382052No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT100.007094712144.976848
GAGCGAA2900.095.0247341
CCGCGTG300.001937219272.488439
TATTCGC350.003563284962.1590421
GTTACGT3750.056.081271
TTACGTG3850.054.6139032
CGAAAGC5900.054.0712783
TACGTGT3900.053.9137273
GTAACTC2750.052.7306633
ACGTGTT4000.052.565884
AGCGAAA6300.050.648111
GTAATGC2300.050.447921
GGTTATA4000.048.9502451
AGTCGGC2000.047.128035
GCGACTA2950.046.697924
GCTACTA4600.045.6992269
GCGAAAG7150.045.6323052
ACTCCGA7050.042.1741031
TCCGAGG8200.041.557553
CAGCAAC9900.041.003557
Produced by FastQC (version 0.11.5)