FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l01_n01_TR_123-7_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l01_n01_TR_123-7_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2666345
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC44280.16607003219763383No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG42780.16044435360015302No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA41870.1570314419176813No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA37890.14210464137236553No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA36380.1364414582509015No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC35870.13452872752775805No Hit
CCCTGTGCCATCCCCCACTGCCACCCTGCTGCACCCCACACACCCACCGC34460.12924058964612606No Hit
CCATCTCAACAGATGCAGAAAAAGCATTTGATAGCGCTGCTACCAAATCA28860.108238056215531No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG27340.10253736857008378No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6900.0106.1449361
GTTACGT5750.068.100911
TACGTGT6200.063.1509933
AGCGAAA11700.061.971542
CGAAAGC12000.061.026474
TTACGTG6500.060.2363362
GCGAAAG13700.057.687813
ACTCCGA10550.050.1761551
GGTTATA4900.045.8767431
TCCGAGG11750.045.6637953
GAGCAAA73850.045.5611271
GCGATTA3500.043.494225145
AGTCGGC2850.043.2496075
GGTCGTT1850.043.1023867
AATGGGT17000.042.2149857
GTAATGC3450.042.0375981
GTCGTCT3650.041.70679145
TATGTGT17050.039.5491073
AAGCAGG105100.039.107567
CAGCAAC15550.038.692617