FastQCFastQC Report
Wed 16 Oct 2024
HNJLWDSXC_l04_n02_F2C_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJLWDSXC_l04_n02_F2C_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38459673
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGATTCCCTGAGTCCGTACCGGTTCCAGTACGGTTGTTTAGATGAGC1240400.3225196428477174No Hit
CTCACCCGGTCCGGACACCATTAGGACTGACAGATTGAAAGCTCTTTCTC882720.22951833209814343No Hit
CTCGAATTGGCCGACGTGGGATCTGTGTTCTTCGGAGTGCAGCGCACCAC753100.195815497443257No Hit
GCACGTCAGAACCGATACGGACTTCCACCAGAGTTTCCTCTGGCTTCATC675330.1755943166755474No Hit
GGGAAACTTCGGAAGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC668380.17378722902818233No Hit
CTCCGGTTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAA662750.17232335802751106No Hit
CGGCAAACTAGATCCGTAACTTCGGGAAAAGGATTGGCTCCAGTGGTTGG661370.17196454062414934No Hit
CGCCCCTATACCCAAGTCTATCGATCGATTTGCACGTCAGAACCGATACG629360.16364153694182476No Hit
CTGAGATCCAGCTATCCTGAGGGAAACTTCGGAAGGAACCAGCTACTAGA601680.15644438786570025No Hit
GTCGGAAACGTCGTCGACTTTCACACCGACGTCACACACACTAGCGTAAC591110.15369605456603855No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGGACGCTTGGCTGCCA530740.13799909323201992No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTTTGACATTGTGAAGAGTCATGA528450.13740366435252843No Hit
GTGGGATCTGTGTTCTTCGGAGTGCAGCGCACCACGGCCCTGTGCGTGTC515760.1341041043172676No Hit
GGCGTTCAGTTATAATCTCTCAAATGGTAGCTTCAGACCATTGATCTGTC515420.13401570002948282No Hit
GCCGCAAGCTCCACGCCTTGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT511520.13300165084606932No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA505100.13133236988260405No Hit
CGGAAACGTCGTCGACTTTCACACCGACGTCACACACACTAGCGTAACTA496910.1292028665974357No Hit
CCCGGACATGGAAGAATTTCAACGGTTTACCGATACCTTTCGGCATAGGA473600.1231419726319566No Hit
CCGCGCTTGTGCTCCGAAGAACACAATCTTTGCATCTCAGTTAGGGAATA445850.11592662267305288No Hit
CAAAGATTGTGTTCTTCGGAGCACAAGCGCGGTAACGCATTCGAACTTGG445060.11572121271025888No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAAGTCTATCGATCGATTTGCAC425640.11067176780208193No Hit
CCCTGAGTCCGTACCGGTTCCAGTACGGTTGTTTAGATGAGCGATCAGGA422060.10974092265423056No Hit
CAGCTTTGCAACCATACTACCCCCGGAACCGAAAGACTTTCGTTTCCGGA411930.10710699490346681No Hit
CTCAGTCGTGATTACCCGCTGAACTTAAGCATATCATTTAGCGGAGGAAA404450.10516210057220196No Hit
CCCGACTCCAAGGAGCAAGCGAACCAACAGTGCCGCTATCCAGAACGGGG401970.10451726929659541No Hit
GGGAAGTCGGCAAACTAGATCCGTAACTTCGGGAAAAGGATTGGCTCCAG389950.1013919177107928No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)