FastQCFastQC Report
Wed 16 Oct 2024
HNJLWDSXC_l04_n01_Sp30_A_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJLWDSXC_l04_n01_Sp30_A_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTGCTTCCTGATCCAATGACGAGGTAGGCTCTTCTGAATGCTGATAA10.8264462809917356No Hit
CAAGTGTCCACCGGGCCGCCCGGGCCCACCCGGTCCACCAGTCTCTGTCT10.8264462809917356No Hit
ATCGATTCTAGAACCAACAAAAAGAACCGAAGTCCTATTACATTATTCCA10.8264462809917356No Hit
AGGATATCCACGGATTGAATGGGCTGTCTTGGATTGGAATACAACAGCTA10.8264462809917356No Hit
GCCCTGTGGAGCTGCGTCTCCTCCAAGTGCTGGTTCGGCGTATCCTTGTG10.8264462809917356No Hit
CAAGAAGGATGTAGTTGATCTAATGAGAGTAGAATTGCGAAAGTGAGCCT10.8264462809917356No Hit
CAAAACATGAAGCCGGCCTTCGTTTGTGAGTGTCTTTGGTGCGGCGAACG10.8264462809917356No Hit
GCCGCCGCCGCCGCCGCCGGTCTGCGAACAACTTCTGGTCGCTGCACATT10.8264462809917356No Hit
TATGTTTAAAAAAAGGGCAATTGTGTAAGGAGAGAATTCATCTATGCTCT10.8264462809917356No Hit
ATGACAACTTCCGTCATTTGGTTCGACAACACCGGCGAGGCGACCGACTC10.8264462809917356No Hit
CTTGAAAAGAGTGTCGTTGGTTTTGCCGCCAACAAATAGATCGGGAAAAG10.8264462809917356No Hit
GGCATACGCAGACGATCACGGCTGACGGACCCCCAAAAATGTTGAAGCAT10.8264462809917356No Hit
CAGTTCGTCCTGGCGGTCCAGAAGCCGAACAACACCCAGACCGACAACTA10.8264462809917356No Hit
AGCCTGAAAAGGACCTGATAACCAGGGCCTTAGATTCCCCAGGGCTGAGG10.8264462809917356No Hit
ACGTCATACAACCCCAACAGAGTGGAGGTTGGAATGCTGAACCAGAACAT10.8264462809917356No Hit
AAACAAATAAAAACAGACCAAACAAACAAAAGAAGTGTTTAACACCAAGA10.8264462809917356No Hit
CACGGAAACCATAGAAAACTCCAATACTCCAGTCAACGAGATCTTAACGA10.8264462809917356No Hit
CGACTTGGAAGCAATAGTAAAACAGGTTATGGACTCAGTGCTGAACAGAG10.8264462809917356No Hit
TATGGAAAAGTTGTAGTCACAACCAGTGTGACCAGAGATCATTATCAGTC10.8264462809917356No Hit
GGAAAGTCAAAAAATAATATATAATATATAGAATATATATATCAACTTTT10.8264462809917356No Hit
CGGCCAAGCATCCTCCGTAGGCGAGACTGACTCTTCTTTGGTTCTTCATC10.8264462809917356No Hit
CCGAAATCGGTCAATTTCACGTGTCCATTTCCGTCGAGCATAATATTTTC10.8264462809917356No Hit
ATTTAGAATTGAAACGAGAAAAAAAATGTATCATAACGAAGAATCAGACA10.8264462809917356No Hit
GAAATCAGAAGATACCAAAAGTCGACTGAATTGTTGATCAGAAAACTTCC10.8264462809917356No Hit
CGCGAATGGCCTTGTTTCTGGTTGTGTTGGCTTGCGAGATGACCAATTCA10.8264462809917356No Hit
CTCTTTTTCATCCATTTCCTTCTCTTCTTGTTTAGCTTGATGTTTATTCC10.8264462809917356No Hit
GATCGGAAGAGCACACGTCTGACCTCCAGTCACATTCAGACAGCTCGTAT10.8264462809917356TruSeq Adapter, Index 27 (97% over 37bp)
ATCGAAATCATCCTCGCTGAGTTCTCCAGCGCGCTTGTTGACATCAATAT10.8264462809917356No Hit
GCCATGGGACCTCTGACAAATTATTTTCACTTGCCAACCCCCACTATTTA10.8264462809917356No Hit
TGTAAATTTGTTTAAGTTATTTGTAGATTCTGGTTATTAGCCCTTTGTCA10.8264462809917356No Hit
GTTTATTTTAATAAAAAATTAACAATAAACCAAATCAACGAATTACATCT10.8264462809917356No Hit
CAGCGAGCCCCGGGAGGGCATGTCCGAGCCCTCGCACATCATCGAATCAA10.8264462809917356No Hit
GTCAGCAAGAAGTTGAAGCTCCTCAGCGGTCTCTTGACGAGGTACAATAT10.8264462809917356No Hit
GTCGAAAGCTTTCATTGGCAAGCCAGCTCCACAATTCAAAACTCAAGCTG10.8264462809917356No Hit
CGACGTTACACTTTTCGACGCAGTTCTCAACTGTATCTCGTGTCGTTGTT10.8264462809917356No Hit
GTTGAAGAGGAGAAAACAGGAAACTTCTATGACAAGAATACCTCTTTCTT10.8264462809917356No Hit
ATTATATCGCATCCCATGGATTTGATGACGATTCGGCAGAGAAACGAAAA10.8264462809917356No Hit
TGAAGGTATCCTTTCCGAGCACTCCGTTGCATGATCCCGATCCGTATTCG10.8264462809917356No Hit
GATGCGTGAGCAGGACGAAGCCAGAGGAAACTCTGGTGGAAGTCCGCATC10.8264462809917356No Hit
CAAAAACTCAACATTTGGACAACAATGACCGAAAAGACAGACATGATCCC10.8264462809917356No Hit
GGGAATAAGAATAAAAGTATAAAAATGTACGGTAGTAATGGGAAAAACAT10.8264462809917356No Hit
CTTATATGGCGTCGGATTCAGATATCGACGATGATCTTGCCGCAGGGTTC10.8264462809917356No Hit
GCGCAAGGGTCACGTCATAGGCTTCTGCCATTCCGGCGATTTTGTAGCAT10.8264462809917356No Hit
CCTGTCCTCATCTTCTTCCTTCAAGTAGGTCATGTAGTCAGCAACGTGCT10.8264462809917356No Hit
TTTGGAGTCTATCAGCTCCGGCACATGAAAGCATTTTGTTGATACGTGTG10.8264462809917356No Hit
TCCTGGTGGTCCTGGTGGTCCTGGTGGTCCGGCGTCTCCTGGCTCTCCTG10.8264462809917356No Hit
GTAAAAGCCGCTGAGGATCTTAAGAGACAACAATTGTTGAAAGAGCAAGA10.8264462809917356No Hit
CGAGAACAATTGAGTTCGTGACATCGCCGAGCAGCCGATTCAGTGTTTGA10.8264462809917356No Hit
AGATAGAAGAAGAGAAAAGGAACGGCTAAACTCACATCTGTCTTGGCTGC10.8264462809917356No Hit
AAATTTTTAAAATACCTGAAAGAAAAGAAAGAAAGTTGTCTGTTCTAAAA10.8264462809917356No Hit
TCGACTCTTCCGGCTATATTTGTACTGTTGACCAATGCAAACTTCATGAC10.8264462809917356No Hit
CCGCTATTCGCACCGCCCACTCATTTGAAGCAGAAGATGATATTTCCGAT10.8264462809917356No Hit
CGTCACGAAGGATACGCCAACACAGGAGTTCAAAGACAATCTGTTAATAT10.8264462809917356No Hit
CGAGACTTTAAAACAGCAGAACAGGGTCGTGCGGGTCTCGAAGCGAATCG10.8264462809917356No Hit
TCCGACTCCAAAGAACAGGAAAACCAAAACTATCGCCGCCCAGAACAGGA10.8264462809917356No Hit
GAGGACGCGGAGGACGTGGTGGAAACCGCAACAACACTCCATTTGATGCT10.8264462809917356No Hit
GTTGCCCACCCCTTTTGGGGTGGTGTCTTACTTGTGGCTAGGTTTGTGGC10.8264462809917356No Hit
CCTCTTTCCACCACTCCCACATGTCTTCCAAATCTTCTTCCTTCTTTGGC10.8264462809917356No Hit
GGTAAGATCCAAGAGAATTCCCTGCATTACCAGGCAGAGACTCTTTTCCC10.8264462809917356No Hit
AGCCAACATAAATCTCTCCAATCATAGAATCAAGATACAATTTCGCAATT10.8264462809917356No Hit
GCCTTTTCCTTTTCTATGTTGTGTTCGTTTTGTTTCTTTCTCCCCACTTT10.8264462809917356No Hit
GTCCAATCGTATCCAGTGCGTACTGAATTCCTGTATTCATTTGGTACGGT10.8264462809917356No Hit
TAGGGCATAAGCTCTCGTGTTACCACGAGATAGAGTTACCTACCGACACC10.8264462809917356No Hit
GGAATCCCAGGAGTACCAGGACACGACGGAAACGACGCCGACGACGCCAA10.8264462809917356No Hit
ATTAAGTTACAAAAAAAAACACACAAAATAAGAAACAAATAATTTCTTAA10.8264462809917356No Hit
GGAAGCTGAGGCAGGAGGATGGCTAGAGCTCAGGGGTTTGAGACTAGCCT10.8264462809917356No Hit
TACATTTTTAAATTCTGTCTATACGTCTCTCCGTATGGCTCTAAATAATG10.8264462809917356No Hit
GAAAGATAAGCAATTGAATCTGAAAATCTTCAAACAATAGCGCTAACAAT10.8264462809917356No Hit
GCGGAGCATCGTTGGGCGTTTTCCAATGGTCAGTGTGTGTCGTTCATTTT10.8264462809917356No Hit
GCGGATTGCCAGCACCGAGTGTCGAATGGACAGTTGATGGCAATCCGCTT10.8264462809917356No Hit
CGGGGGGCCCACTGCGTAGATATAATCCTTTGCCATTTCACCGATATACT10.8264462809917356No Hit
CGCCCGTCTTCTGTTTCCCATGTGTGCCAACCTATATGGGAATCTGGCCG10.8264462809917356No Hit
TCAGTAATTAGAAGACCAGGATCATCAATGTTTTTGTAATCTGTCAAATT10.8264462809917356No Hit
CTTCTCTTTTCCAAATCATATATCTCATCTTCAAGTTTGACAATTCTTGC10.8264462809917356No Hit
AAAAATGCCACCAATGTTCGACCCAACTGAGATTAAGATTGTCTACCTTC10.8264462809917356No Hit
GAATATCGTTTCTGGAGAATTAAACAATGCCGCAGCAAAGAAGTTGGATT10.8264462809917356No Hit
GAGGCTTTTTCTAAGGAAAATATAAAAAACAAATGTCGGAGTGGTTGTGC10.8264462809917356No Hit
GGAGATGCGGGTGCTAAAGGTTTTCTGTGGTTCTGCTCTGGGCTTACTAG10.8264462809917356No Hit
TCCCAATAACAAGTCAACACAAGGGAAAAACTAACCTCACTAACCACGCT10.8264462809917356No Hit
CAACATGCTCATCGTCACCGTCTTCTCAATATCTCTCGACGTGGTCCGAT10.8264462809917356No Hit
GTGGCAAGGATGACAATGCCAAGAAGAAGAAAGGCTCAGCACGTAAACAC10.8264462809917356No Hit
AATAAACGCAGAGTAAAATAAGAATAGCAAAATTGCTTATATGGACGAAA10.8264462809917356No Hit
GATTGAAAGCTCTTTCTCGATTCGGTGGTTGGTGGTGCATTGCCGTTCTT10.8264462809917356No Hit
AGCAACCTAGTCATGAGATCTCCTGATGCTAAATCGGTAGATCCGGTAAA10.8264462809917356No Hit
AGAAAAGGAGAAAGGAGATTATGAATACATTCCTGATGATGAAAATTACG10.8264462809917356No Hit
GTGAATTCTTCAACAAGTTGTATTCGATAATCGTTTATGATGACACGTGG10.8264462809917356No Hit
GTGGTGGAGGTGGTGCGACTGGGGGAGCAGCATATGATCCAGCTCCTCCG10.8264462809917356No Hit
GCGGCAGTTTTGATTTTGAATGAAAGTTGTCGGAGAAAATAAAAACGGAA10.8264462809917356No Hit
GACTCTTTCACCTTCTCCTAAAAGAGGGACAGGTATTCGAAGAGAAGCAC10.8264462809917356No Hit
GCTGGACAAGATGGATATCCAGGACAACCAGGACAAGATGGACAACAAGG10.8264462809917356No Hit
CCCCTCCTTCTTTTTGGCGGCAAGCAACGAGACCTTGTCAATTAGTTGTC10.8264462809917356No Hit
ACCTTACCAAGAAGTACTTGAAGAGAAACTCACTCCGTGATTGGCTCCGC10.8264462809917356No Hit
GGAGAGCATAGCACTCGTTTGATCAAGCTTACATCGTCAGATTTGGAACC10.8264462809917356No Hit
ATTTTTATTTTTTATTTTTTATTTTTTTTGAGACAGAGTCTCGCTCTGTC10.8264462809917356No Hit
CGAGAACCTTAAAAAAAATTCTCACAAAGAGCAACACACTTATAACCAAA10.8264462809917356No Hit
ATCTCTTTCAACAATTCCATCGAATTCGACTCTTTTATTAACAATTGGTG10.8264462809917356No Hit
GCCTGTGGTGGAAAAGGAAAATCTTCACATAAAAACTAGATGGAAGCCTT10.8264462809917356No Hit
GCCCCGGGTGCCACTGGTGGGACTATCAAACTGTCAGATGACAAAGCTGG10.8264462809917356No Hit
TCACAACTAAACTGTTTCACAAATAGAAATACAATATCGATTAGTTCAAT10.8264462809917356No Hit
GGTCGACACATAATCGCGTCCAATGACGAGCTGGCACACAGGGGTGGTGG10.8264462809917356No Hit
TAAAAGGAGCCACAAATAAGGTTAGAAGGTGTCTTTGCTAAGAGTGACCA10.8264462809917356No Hit
ATGACCTGGAAATATGTACACCAACAACAACTTGGCTTTTAAAACAAGCA10.8264462809917356No Hit
GACCAATTATAGTTAAGTGCTGGTAGTAGTACGAAAAGCCCTAAAGAAGA10.8264462809917356No Hit
CAAGTCTGATGGTCCTGTCGGTGGTGGTGGCGGTCTATGATGATGATGAC10.8264462809917356No Hit
GAAAGAACTCAAAGAAATAATCGACGAATGAATTACTACAATTCGAAAAT10.8264462809917356No Hit
TTTTGAAACAAAAATTTATTAGCAACAAAAACACATATAATCCTCTACAT10.8264462809917356No Hit
CGCCAAACTGTTGAGCTCGTCAAGAGAAATGTCAGATGAGACGCATCAAA10.8264462809917356No Hit
CCAAAGAAGTATCAAAATAATCGCCATAAGGATGGAGGGGCTTCTTAAGG10.8264462809917356No Hit
ATCATATGTGGCTGATATTAATGCCGATATTGGAAAGACAACAATTGTGT10.8264462809917356No Hit
GTTCATGGAAAATTTTTCTCATGTAGAATGCTTCTTTACTATGAGGTGTG10.8264462809917356No Hit
ATTAAGATCATCATTAATGATAACTTTATCAAATAACGTGGGATCCTTTT10.8264462809917356No Hit
CGGTATACTATTCGGTGGACTATCTGTCGATGTTCCACTTGCTGTTCTCG10.8264462809917356No Hit
AAAAGAAAGGAAGACGAAATACAAGACAACCGATCAGAGCATTGAATAGA10.8264462809917356No Hit
AAATAATACATGCATGTTGCACAAGTTCGCGAGCACTCCGACACCGGCCG10.8264462809917356No Hit
GGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAG10.8264462809917356No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTTCCATCGCGTATG10.8264462809917356TruSeq Adapter, Index 7 (97% over 36bp)
GTTTGTGTGCGTGAGCCAAATATGAATTTTGGTTGAGAATAAGAAAAATG10.8264462809917356No Hit
CCAATGCTTCTTGTTCTCTGACCTTTCTCTTGGTGTCGTTTGAAACATAA10.8264462809917356No Hit
GCGGCTGCCAATTGATTTGGTTGTGTCAATTCCATTTTAATGGATGCAAA10.8264462809917356No Hit
CGAAATGATACGAAGCGCTTCTTTGCAAGCGTGATACATTCGAAGCATCT10.8264462809917356No Hit
ATTTTGAGCAGGTCTTTTCGATGTCCTTTCCAATTGAGTCTGGGATGAGC10.8264462809917356No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)