FastQCFastQC Report
Wed 16 Oct 2024
HNJLWDSXC_l04_n01_F2D_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJLWDSXC_l04_n01_F2D_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20077034
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6153923.065153946544096No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT1017270.5066834075192581TruSeq Adapter, Index 7 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA874030.4353382078249208No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCGCGTAT611360.30450712988781115TruSeq Adapter, Index 7 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTA206400.10280402971873236TruSeq Adapter, Index 7 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)