FastQCFastQC Report
Mon 31 Aug 2020
HNJLTDRXX_l02_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJLTDRXX_l02_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34534914
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2697010.7809517058591777No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA616180.1784223351475553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCACG92450.036.7382931
GATCAAC246500.034.4173661
GATCCAC146000.034.2488821
GATCCAG142600.034.200291
GATCCGC64300.032.505851
GATCATG175950.031.6775781
GATCCGA135350.031.3137191
GATCGCG47950.030.8760411
GATCCGG79300.030.8415871
GATCCTG115750.030.158111
GATCGTG121200.029.9995771
GATCCCG69300.029.7416551
GATCCGT99050.028.4287191
GATCGCA84900.028.0379451
GATCCCA110100.027.7507551
GATCACA249900.026.4274461
GATCCTT245850.025.4753781
GATCCAT227250.025.3249931
GATCCCT79900.025.2509691
GATCCAA266750.025.0845641
Produced by FastQC (version 0.11.5)