FastQCFastQC Report
Mon 31 Aug 2020
HNJLTDRXX_l02_n02_DJ61-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJLTDRXX_l02_n02_DJ61-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences273560820
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCACG1236100.023.5301171
GATCCGC813700.022.1889111
GATCAAC3160600.021.8193171
GATCGCG523050.021.1997181
GATCCAC1940400.020.9545971
GATCCCG919400.020.7666441
GATCCGG1009100.020.596281
GATCCTG1542800.020.4799441
GATCGTG1460150.020.3072361
GATCCTT3136250.019.6864221
GATCCGA1726650.019.4970991
GATCCAG1915900.019.1961331
GATCATG2338150.018.4420951
GATCGCA1196550.017.9579811
GATCCCA1567100.017.8979281
GATCCTC2169750.017.7362541
GATCGTC1911800.017.6278651
GATCCGT1346450.017.3546411
GATCACC1466300.017.0744291
GATCCCT1074400.016.852121