FastQCFastQC Report
Mon 31 Aug 2020
HNJLTDRXX_l02_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJLTDRXX_l02_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34534914
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA715460.2071700540502287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCACG97850.038.4993061
GATCCAC157600.037.9234731
GATCCGG80550.037.1445351
GATCAAC258850.037.0832751
GATCCCG71000.036.9369161
GATCCTG114650.035.9541171
GATCGTG115850.035.3940931
GATCCGC67450.034.5847241
GATCATG173400.033.1309971
GATCCAG149600.032.4938621
GATCCGA132100.032.191831
GATCCCA122700.031.174521
GATCACA258700.030.1598831
GATCGCA92050.029.8281151
GATCAAG249150.028.1756081
GATCACC122050.027.6604041
GATCCTT251150.027.287791
GATCCAT236550.027.0119531
GATCGCC68300.026.94161
GATCGCT79450.026.716781
Produced by FastQC (version 0.11.5)