FastQCFastQC Report
Mon 31 Aug 2020
HNJLTDRXX_l02_n01_JK06-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJLTDRXX_l02_n01_JK06-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222596129
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCAAC3882150.044.748721
GATCACG1482600.042.49511
GATCGTG1743300.040.1132661
GATCATG2656100.039.2324031
GATCGCG621550.037.059321
GATCCAC2547650.035.9405441
GATCCTG1837400.034.439331
GATCCGA2073250.034.016241
GATCACA4016500.033.0569231
GATCGCA1381050.032.3807681
GATCCAG2432500.032.2483021
GATCCGG1206350.032.1161581
GATCCAA4162350.031.1145081
GATCCCA1870850.030.8540741
GATCCCG1098800.029.1542431
GATCCAT3956400.028.9927541
GATCCTA1594850.028.503761
GATCATA3390550.028.2163311
GATCGTT2811900.027.5205761
GATCAAG3900650.026.8951821