FastQCFastQC Report
Mon 31 Aug 2020
HNJLTDRXX_l02_n01_DJ61-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJLTDRXX_l02_n01_DJ61-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences273560820
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCACG1207300.024.6239091
GATCGCG509100.022.7935091
GATCAAC3164200.022.0429611
GATCCGC806150.021.987691
GATCGTG1388450.021.6045171
GATCCAC1959700.021.3207531
GATCCCG918950.021.1434121
GATCCTG1507800.020.5581991
GATCCGG973950.020.4185561
GATCCGA1695650.020.0129491
GATCCAG1887050.019.512731
GATCCTT3157250.019.3533081
GATCATG2283250.018.7632371
GATCCGT1314400.018.5177691
GATCCCA1589400.018.2433191
GATCGTC1820400.018.1713621
GATCCTC2210550.017.9203151
GATCACA3176250.017.682551
GATCCCT1095700.017.640051
GATCGCA1190900.017.5209981