FastQCFastQC Report
Mon 31 Aug 2020
HNJLTDRXX_l02_n01_DJ60-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJLTDRXX_l02_n01_DJ60-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338598650
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCAAC4646800.041.365181
GATCACG1748700.039.657681
GATCCAC2950700.038.0462381
GATCGCG751000.036.301641
GATCCGC1247300.033.195791
GATCCAG2914700.032.9448851
GATCGTG2039300.032.3491741
GATCATG3231200.032.2968831
GATCCTG2260650.031.841321
GATCCGG1518650.031.7153591
GATCCGA2539200.030.2277471
GATCCCG1367400.030.2165221
GATCACA4462600.030.0823881
GATCGCA1640750.029.6292511
GATCCCA2305250.029.438281
GATCGTC2601700.028.7322031
GATCCAT4388950.028.4968241
GATCACC2164200.028.2771741
GATCCAA5167350.027.7663081
GATCATC5577750.027.7452431
Produced by FastQC (version 0.11.5)