FastQCFastQC Report
Mon 31 Aug 2020
HNJLTDRXX_l02_n01_DJ59-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJLTDRXX_l02_n01_DJ59-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199868191
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC563700.068.680231
GATCCGG615850.063.465021
GATCCCG644450.061.0086441
GATCTGC1012750.058.2652851
GATCACG832100.055.3738251
GATCCAG1177150.055.0878071
GATCAGC1108350.054.1950381
GATCCTG1035650.053.2863241
GATCGCG427150.051.634171
GATCGCC584750.051.200081
GATCCGA1136050.050.9392131
GATCGGC777950.050.0265051
GATCTGG943850.049.624521
GATCCGT826600.049.6091271
GATCGGG535950.048.7168731
GATCCAC1303850.048.477581
GATCGTG1009800.048.387411
GATCTTG1547900.047.9141851
GATCTCC1211000.046.9841
GATCTCG1193650.046.9013371