FastQCFastQC Report
Mon 31 Aug 2020
HNJLTDRXX_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJLTDRXX_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35486243
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3243100.9139034526703772No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410540.11568990270398587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCACG102200.036.010761
GATCCAC149100.034.1770631
GATCAAC250100.033.8132931
GATCCTG124750.032.5853771
GATCCGG86950.031.3066751
GATCATG179350.030.8005541
GATCGCG53550.030.3643361
GATCGTG124800.029.722241
GATCCCG73500.029.4309431
GATCCGA129900.029.2258931
GATCCAG150350.028.8234631
GATCCCA120200.028.685631
GATCCGC66950.028.5154631
GATCGCA89000.027.6493511
GATCCTT251100.026.653821
GATCACA251050.026.0519281
GATCCGT107950.025.9561271
GATCACC126550.025.0091861
GATCGCC67350.025.0049291
GATCAAG252200.024.8106081
Produced by FastQC (version 0.11.5)