FastQCFastQC Report
Mon 31 Aug 2020
HNJLTDRXX_l01_n02_DJ61-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJLTDRXX_l01_n02_DJ61-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274463200
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCACG1226050.023.825731
GATCGCG556150.022.2103861
GATCCGC820100.022.0000081
GATCAAC3191700.021.874671
GATCCAC1942150.021.1879431
GATCCTG1549600.020.3356821
GATCCCG913300.020.3350541
GATCGTG1473550.020.0504781
GATCCGG1005150.019.98591
GATCCTT3156750.019.6246871
GATCCGA1762200.019.5708521
GATCCAG1903550.019.223261
GATCATG2338250.018.32811
GATCGCA1196150.017.9170421
GATCCTC2178700.017.8115311
GATCGTC1905350.017.491041
GATCCGT1360000.017.4233931
GATCCCA1571600.017.4141791
GATCACC1463750.017.4081421
GATCACA3173650.017.1030271