FastQCFastQC Report
Mon 31 Aug 2020
HNJLTDRXX_l01_n02_DJ59-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJLTDRXX_l01_n02_DJ59-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200391136
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC578550.065.756961
GATCCGG631050.061.160371
GATCCCG651100.058.9983371
GATCGCG391450.057.585181
GATCTGC1016500.056.8175051
GATCACG840350.054.926571
GATCCAG1197600.054.812851
GATCAGC1120750.052.523211
GATCCTG1041000.051.659841
GATCTCG1090250.050.6575471
GATCGCC589250.050.1772651
GATCCGA1147750.050.016671
GATCTGG974600.049.557251
GATCCGT837700.049.0531541
GATCCAC1297350.048.3943561
GATCTTG1572250.047.8123251
GATCGTG1040900.047.4465561
GATCGGC798150.047.2556531
GATCTCC1184100.045.9928171
GATCCTC1411400.045.897851