FastQCFastQC Report
Mon 31 Aug 2020
HNJLTDRXX_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJLTDRXX_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35486243
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA462450.1303181066533304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC67000.040.1168671
GATCAAC261150.039.643331
GATCGTG118300.037.6020471
GATCACG100150.037.4719851
GATCCAC162950.036.146321
GATCCCG77750.035.7815171
GATCCGG79200.035.58381
GATCCTG122050.034.0723421
GATCCGA133650.033.8254471
GATCATG178100.032.78681
GATCCAG156900.031.7682551
GATCCCA126500.030.1247271
GATCGCA97350.029.5448971
GATCCGT109100.029.0855481
GATCACA275800.028.5537511
GATCACC129600.027.3916991
GATCAAG262250.027.3770141
GATCCTT272100.027.3711151
GATCGCC66950.027.2695431
GATCGAC125350.026.81771
Produced by FastQC (version 0.11.5)