FastQCFastQC Report
Mon 31 Aug 2020
HNJLTDRXX_l01_n01_JK06-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJLTDRXX_l01_n01_JK06-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222215701
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCAAC3866700.044.5177541
GATCACG1505850.042.0452541
GATCGTG1735600.040.520261
GATCATG2658000.039.664711
GATCGCG620350.036.265151
GATCCAC2498450.035.9509051
GATCCGA2105300.034.1901471
GATCCTG1833300.033.601851
GATCACA4004950.033.2149851
GATCCGG1210300.033.095311
GATCGCA1372150.032.0404661
GATCCAG2429050.032.006841
GATCCAA4162500.031.0744421
GATCCCA1879600.030.9413491
GATCCCG1105900.029.733781
GATCCAT3959650.028.741271
GATCCTA1603850.028.6010041
GATCATA3386450.028.4019931
GATCGTT2781450.027.6779041
GATCGTA1831500.026.7605931
Produced by FastQC (version 0.11.5)