FastQCFastQC Report
Mon 31 Aug 2020
HNJLTDRXX_l01_n01_DJ61-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJLTDRXX_l01_n01_DJ61-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274463200
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCACG1218850.024.2293171
GATCGCG516800.022.7199841
GATCCGC822100.022.6015471
GATCAAC3162850.022.2673741
GATCGTG1398250.021.9868131
GATCCGG977000.021.2599451
GATCCTG1538800.021.2416921
GATCCAC1972150.020.972351
GATCCCG924200.020.7167511
GATCCAG1909100.020.0732541
GATCCTT3188150.019.5770071
GATCCGA1721550.019.4796831
GATCATG2285650.018.7811551
GATCGTC1860750.018.4550691
GATCCTC2195700.018.2359521
GATCGCA1199800.018.1461931
GATCCCA1586800.018.0579531
GATCCGT1325450.017.8650191
GATCCCT1095650.017.6602841
GATCACA3206400.017.5859721