FastQCFastQC Report
Mon 31 Aug 2020
HNJLTDRXX_l01_n01_DJ60-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJLTDRXX_l01_n01_DJ60-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339353898
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCAAC4627200.041.5651
GATCACG1764800.039.813241
GATCCAC2965050.037.886131
GATCGCG730900.036.6148531
GATCCGC1258850.033.5188141
GATCGTG2049450.032.7760281
GATCCAG2950600.032.145481
GATCATG3247050.032.0271871
GATCCTG2276900.032.0214231
GATCCGG1526300.031.6510751
GATCCGA2582200.030.8671651
GATCCCG1354800.030.3954871
GATCGCA1665300.030.263471
GATCACA4464050.030.221741
GATCCCA2308950.029.104941
GATCGTC2599000.028.7477681
GATCACC2162650.028.1260851
GATCCAT4379150.027.9971791
GATCCAA5222300.027.9321231
GATCGCT1427850.027.7432941