FastQCFastQC Report
Mon 31 Aug 2020
HNJLTDRXX_l01_n01_DJ59-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJLTDRXX_l01_n01_DJ59-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200391136
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC577200.068.340841
GATCCGG613050.063.2441981
GATCCCG653700.060.287711
GATCTGC1011600.056.5015221
GATCACG841100.055.77121
GATCCAG1190500.054.6023831
GATCCTG1028750.054.509171
GATCAGC1103500.053.715151
GATCGCG430400.052.877181
GATCCGA1130500.051.2903371
GATCGCC577150.050.201381
GATCTGG955750.050.052291
GATCCGT839600.049.953781
GATCCAC1313850.049.476071
GATCGGC789500.049.1553081
GATCGTG1000000.048.5772321
GATCTTG1568000.047.761031
GATCGGG533900.047.4353331
GATCCTC1401350.047.225211
GATCTCC1218500.046.5981181