FastQCFastQC Report
Sat 9 Sep 2017
HNJJKAFXX_n02_733.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJJKAFXX_n02_733.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6222310
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG4705437.5621915333694405Illumina Single End PCR Primer 1 (100% over 58bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG639151.027190866414563No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAGGGGGG367510.5906327392881422Illumina Single End PCR Primer 1 (100% over 58bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG86180.13850161756646648Illumina Single End PCR Primer 1 (98% over 58bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG620650.058.57684366
AAGGGGG626750.058.01820467
AGGGGGG639600.056.9825268
AAAAGGG646850.056.39628665
AAAAAGG684750.053.6166364
CACACGA29600.049.531219
ACACGAG29950.049.5282820
AAAAAAG758050.049.18281663
AGAGCAC79050.048.74139815
GAGCACA76550.047.8101816
GCACACG42550.045.1557218
CACGAGA34100.043.09529521
AGCACAC53250.041.8468817
GGTCGGA20150.040.789182
TAAAAAA1062750.039.3714758
TTAAAAA1066100.038.67495757
AAGAGCA134000.038.378214
CATTAAA1068650.037.9883455
ATTAAAA1093100.037.6849556
GTGACTG28450.037.4109271
Produced by FastQC (version 0.11.2)