FastQCFastQC Report
Sat 9 Sep 2017
HNJJKAFXX_n02_733-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJJKAFXX_n02_733-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7251156
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG5740887.917192789673812Illumina Single End PCR Primer 1 (100% over 58bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG716710.988407917303117No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAGGGGGG462610.6379810336448423Illumina Single End PCR Primer 1 (100% over 58bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG104900.14466658833432905Illumina Single End PCR Primer 1 (98% over 58bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG760050.059.04849666
AAGGGGG768000.058.39232367
AGGGGGG781100.057.5543868
AAAAGGG791550.056.90789465
AAAAAGG842200.053.85009864
CACACGA37400.050.2707319
AAAAAAG925300.049.80822863
AGAGCAC97800.049.16683215
GAGCACA94800.049.15880616
ACACGAG38600.048.43979320
GCACACG52200.048.3737618
AGCACAC64100.046.60335517
GGTCGGA25950.045.6413042
CACGAGA44400.041.87926521
GTGACTG32300.040.1168941
AAGAGCA160350.039.68457414
TAAAAAA1341350.038.33043358
TGACTGG34500.037.8332822
TTAAAAA1365000.037.18911457
AGGTCGG32100.037.029851
Produced by FastQC (version 0.11.2)