FastQCFastQC Report
Sat 9 Sep 2017
HNJJKAFXX_n02_666.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJJKAFXX_n02_666.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8759552
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG128453014.664334431715229Illumina Single End PCR Primer 1 (100% over 58bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAGGGGGG1084941.2385793246047285Illumina Single End PCR Primer 1 (100% over 58bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG719230.8210808041324488No Hit
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG241850.2760985949966391Illumina Single End PCR Primer 1 (98% over 58bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAATGGGGGG157650.17997495762340357Illumina Single End PCR Primer 1 (100% over 58bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGG92640.10575883332846246Illumina Single End PCR Primer 1 (100% over 58bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG1633950.059.66136666
AAGGGGG1640150.059.36884767
AGGGGGG1657850.058.79395368
AAAAGGG1664950.058.7477565
CACACGA94700.057.9551719
AAAAAGG1724000.057.07580664
ACACGAG96750.056.72847720
AGAGCAC239750.054.51526315
GAGCACA233750.054.2030716
AAAAAAG1858600.053.70639463
GCGGCGC25300.052.586271
CACGAGA105400.052.0395421
GCACACG137450.051.7491818
AGCACAC161600.051.72126817
GGTCGGA51700.051.43592
AAGAGCA336450.050.65405314
CACGTAG32300.048.27395221
GCACAAG73250.047.13961418
GTGACTG58850.046.50621
TGACTGG58850.046.419042
Produced by FastQC (version 0.11.2)