FastQCFastQC Report
Sat 9 Sep 2017
HNJJKAFXX_n02_666-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJJKAFXX_n02_666-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7658450
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG120353915.715177353119754Illumina Single End PCR Primer 1 (100% over 58bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAGGGGGG844421.102599089894169Illumina Single End PCR Primer 1 (100% over 58bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG598830.7819206236248849No Hit
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG209880.2740502320965731Illumina Single End PCR Primer 1 (98% over 58bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAATGGGGGG153080.199883788495061Illumina Single End PCR Primer 1 (100% over 58bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGG92030.12016791909590059Illumina Single End PCR Primer 1 (100% over 58bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG1527950.060.54807766
AAGGGGG1532600.060.33814267
AGGGGGG1547800.059.85077368
AAAAGGG1557400.059.5670965
AAAAAGG1607650.058.03108264
CACACGA71000.055.24435419
AAAAAAG1739550.054.49740663
ACACGAG72700.054.0003320
AGAGCAC190800.053.91285315
GAGCACA185200.053.55075516
GCGGCGC23100.051.748831
GGTCGGA40150.051.5152782
AGCACAC120250.050.1740517
AAGAGCA276050.049.72774514
CACGAGA79650.049.6342821
GCACACG102450.049.49710518
GTGACTG56350.048.19091
GCACAAG67350.047.8391818
TGACTGG57450.047.662622
GAGCAAG59950.045.45761516
Produced by FastQC (version 0.11.2)