FastQCFastQC Report
Sat 9 Sep 2017
HNJJKAFXX_n02_395-lg.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJJKAFXX_n02_395-lg.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11829233
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG2213091.8708651693647425Illumina Single End PCR Primer 1 (100% over 58bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1652591.3970390134339226No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA248100.053.9191616
AGAGCAC257850.053.7530415
CACACGA80850.052.6527619
GCACACG104900.052.38533818
AAAGGGG334200.051.88419366
ACACGAG83400.051.00150720
AAGGGGG343250.050.4764567
CACGAGA84000.050.4725121
AAGAGCA399050.048.42079514
GCACAAG123950.047.72951518
AGCACAC138800.046.0777817
AGGGGGG380750.045.96763268
CGTATCA426200.045.25395250
AAAAGGG388200.044.96025565
GTATCAT431550.044.5351351
AGCACAA137800.043.03263517
ACGAGAT102200.041.5841122
GAGCAAG109000.041.36292316
CGAGATC112500.040.6281723
CATTAAA484650.040.39041555
Produced by FastQC (version 0.11.2)