FastQCFastQC Report
Sat 9 Sep 2017
HNJJKAFXX_n02_395-lg-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJJKAFXX_n02_395-lg-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5276746
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG4852279.195572422853024Illumina Single End PCR Primer 1 (100% over 58bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG946641.7939843987184525No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAGGGGGG368340.6980438323163556Illumina Single End PCR Primer 1 (100% over 58bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG61060.11571525330194025Illumina Single End PCR Primer 1 (98% over 58bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAATGGGGGG60370.11440762924726716Illumina Single End PCR Primer 1 (100% over 58bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA195250.061.86989216
AGAGCAC201050.061.71673615
AAGAGCA314050.058.54890414
GCACACG76050.058.5130818
AAGGGGG633600.058.47290467
AAAGGGG634400.058.4366966
GCACAAG97000.058.3934218
AGCACAC91750.057.97517
AGGGGGG642450.057.81781468
CACACGA61150.057.20376219
AAAAGGG653050.056.8529265
AGCACAA102600.055.20625317
ACACGAG63400.055.06535720
GAGCAAG89600.054.93766416
CACGAGA64000.054.44079621
AAAAAGG689100.054.2242264
CACAAGA110200.051.46397419
AAAAAAG739000.051.4121963
AGAGCAA99650.050.29848515
GTGACTG43100.049.139911
Produced by FastQC (version 0.11.2)