FastQCFastQC Report
Sat 9 Sep 2017
HNJJKAFXX_n02_395-hg.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJJKAFXX_n02_395-hg.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16296796
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2753501.6895959181178926No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG1920061.1781825090036102Illumina Single End PCR Primer 1 (100% over 58bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG299300.050.89016766
AAGGGGG301500.050.66808367
CGTATCA347450.047.7260350
AGAGCAC138100.045.6111715
CCGTATC367500.045.0591249
GCACACG55200.044.80779318
GAGCACA135750.044.24094816
GCCGTAT377600.044.01833748
AGGGGGG359650.043.1763668
AAAAGGG357050.042.9589565
GTATCAT389750.042.6898551
CGCCGTA394100.042.2805747
TCGCCGT412100.040.2658946
CATTAAA421750.040.0016555
ATCATTA440800.038.1390353
GTCGCCG438850.037.87426445
GGTCGCC441000.037.7442644
TCATTAA449100.037.33510654
TATCATT448950.037.24596452
AGCACAC78000.036.5747817
Produced by FastQC (version 0.11.2)