FastQCFastQC Report
Sat 9 Sep 2017
HNJJKAFXX_n02_395-hg-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJJKAFXX_n02_395-hg-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7626741
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2079492.7265774463824064No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG1843422.41704812055372Illumina Single End PCR Primer 1 (100% over 58bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG265700.055.01539266
AAGGGGG271550.054.03387567
CGTATCA308200.051.58864650
AGGGGGG292050.050.97396568
GTATCAT319600.049.63270651
AAAAGGG298800.049.0714465
CCGTATC324850.048.85117349
GCCGTAT334150.047.71900648
AGAGCAC87000.047.2222815
CATTAAA346550.046.4019355
GAGCACA83950.046.34895716
ATCATTA349150.045.75905653
TATCATT346700.045.74378652
CGCCGTA349400.045.60722747
TCATTAA355550.045.05240654
GCACACG36600.044.769118
TCGCCGT360450.043.9602446
AAAAAGG340400.043.5100964
CACACGA29100.042.43976619
ATTAAAA390850.041.82338756
Produced by FastQC (version 0.11.2)