FastQCFastQC Report
Sat 9 Sep 2017
HNJJKAFXX_n02_395-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJJKAFXX_n02_395-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8555263
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG3346893.9120831235696674Illumina Single End PCR Primer 1 (100% over 58bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG936281.094390669228988No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAGGGGGG262710.30707413670392136Illumina Single End PCR Primer 1 (100% over 58bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG467600.056.2841866
GAGCACA167800.055.8524316
AGAGCAC175600.055.5319815
AAGGGGG473750.055.51775467
CGTATCA538100.054.25842750
GTATCAT544150.053.42343551
GCACACG74750.053.34716418
AGGGGGG499800.052.9144168
AAAAGGG510400.051.76815465
CATTAAA572100.051.1926755
ATCATTA571100.051.06393453
CCGTATC572050.050.9489849
AGCACAC90500.050.84688617
TATCATT572350.050.744252
CACACGA60800.050.4400619
TCATTAA581000.050.16472254
GCCGTAT591350.049.5253448
ACACGAG63350.048.51862720
ATTAAAA624150.048.03598456
CACGAGA64000.048.02557821
Produced by FastQC (version 0.11.2)