FastQCFastQC Report
Sat 9 Sep 2017
HNJJKAFXX_n02_395-14-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJJKAFXX_n02_395-14-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14234930
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG10105217.098882818531598Illumina Single End PCR Primer 1 (100% over 58bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4619093.244898288927308No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAGGGGGG742980.5219414496593942Illumina Single End PCR Primer 1 (100% over 58bp)
GGGTTTAGAGCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGAATGTTACCGCAATAGTAATTGAACTTAGTA261660.18381544552730503No Hit
TAAGGCAGAGCGACAGAGAGGGCAAAAGAAAATAAAAGTAAGATTTTAGTTTGTAATGGGAGGGGGGGTTTAGTC143170.10057653954041221No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG1327250.058.8516866
AAGGGGG1329200.058.75038567
AAAAGGG1381800.056.67091865
AGGGGGG1422950.055.1300468
GAGCACA300500.054.83354616
AGAGCAC314700.054.57378415
CGTATCA1574500.054.26013650
GTATCAT1574000.054.03457351
AAAAAGG1471450.053.5600164
CATTAAA1603400.053.48581755
ATCATTA1609650.053.04628853
TCATTAA1614700.052.88307654
TATCATT1613250.052.73637452
CCGTATC1634300.052.13825649
GCACACG137050.052.07554618
ATTAAAA1691100.051.28180356
GCCGTAT1672200.051.1071648
TTAAAAA1709600.050.8136557
CACACGA99600.050.636119
ACACGAG100250.050.30832320
Produced by FastQC (version 0.11.2)