FastQCFastQC Report
Sat 9 Sep 2017
HNJJKAFXX_n02_131.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJJKAFXX_n02_131.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6515576
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG1501272.3041247619550442Illumina Single End PCR Primer 1 (100% over 58bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG238050.36535526559739306No Hit
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG122930.18867096324254373Illumina Single End PCR Primer 1 (98% over 58bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG253400.053.5596466
AAGGGGG254200.053.44538567
AGGGGGG263800.051.8278768
AAAAGGG279350.048.8193465
GGTCGGA28550.047.7784232
AAAAAGG316000.043.55002664
AGGTCGG31550.042.1686361
CCGTATC386400.038.2057549
CGCCGTA388100.038.1542547
CGTATCA388500.038.07476450
GCCGTAT390950.037.81433548
TCGCCGT391200.037.67465646
GTCGCCG398750.037.12570645
GTATCAT396850.037.04906551
AAAAAAG381350.037.0278863
GGTCGCC400150.036.9866244
CATTAAA404800.036.75805355
GTGGTCG410150.036.08345442
CTCGGTG411050.035.8528338
TGGTCGC413650.035.76338243