Basic Statistics
Measure | Value |
---|---|
Filename | HNJJKAFXX_n02_131.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 6515576 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 41 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG | 150127 | 2.3041247619550442 | Illumina Single End PCR Primer 1 (100% over 58bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 23805 | 0.36535526559739306 | No Hit |
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG | 12293 | 0.18867096324254373 | Illumina Single End PCR Primer 1 (98% over 58bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AAAGGGG | 25340 | 0.0 | 53.55964 | 66 |
AAGGGGG | 25420 | 0.0 | 53.445385 | 67 |
AGGGGGG | 26380 | 0.0 | 51.82787 | 68 |
AAAAGGG | 27935 | 0.0 | 48.81934 | 65 |
GGTCGGA | 2855 | 0.0 | 47.778423 | 2 |
AAAAAGG | 31600 | 0.0 | 43.550026 | 64 |
AGGTCGG | 3155 | 0.0 | 42.168636 | 1 |
CCGTATC | 38640 | 0.0 | 38.20575 | 49 |
CGCCGTA | 38810 | 0.0 | 38.15425 | 47 |
CGTATCA | 38850 | 0.0 | 38.074764 | 50 |
GCCGTAT | 39095 | 0.0 | 37.814335 | 48 |
TCGCCGT | 39120 | 0.0 | 37.674656 | 46 |
GTCGCCG | 39875 | 0.0 | 37.125706 | 45 |
GTATCAT | 39685 | 0.0 | 37.049065 | 51 |
AAAAAAG | 38135 | 0.0 | 37.02788 | 63 |
GGTCGCC | 40015 | 0.0 | 36.98662 | 44 |
CATTAAA | 40480 | 0.0 | 36.758053 | 55 |
GTGGTCG | 41015 | 0.0 | 36.083454 | 42 |
CTCGGTG | 41105 | 0.0 | 35.85283 | 38 |
TGGTCGC | 41365 | 0.0 | 35.763382 | 43 |