FastQCFastQC Report
Sat 9 Sep 2017
HNJJKAFXX_n02_131-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJJKAFXX_n02_131-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25268479
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5882392.327955711145099No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG2900751.147971747725694Illumina Single End PCR Primer 1 (100% over 58bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG459600.049.64415767
AAAGGGG465400.048.876666
AGAGCAC239500.047.5577815
GCACACG101050.046.2898218
CGTATCA542950.045.7707550
GAGCACA243900.045.6536216
AGGGGGG510100.045.60213568
CCGTATC573250.043.27039349
GCCGTAT595900.042.1641148
AAAAGGG561800.040.7111665
GTATCAT616850.040.3175951
CGCCGTA632500.039.75729847
AGCACAC142700.038.72528517
CATTAAA654750.038.5765455
TCGCCGT662800.037.72578446
ACACGAG93450.037.61643620
CACACGA96550.036.65862319
ATCATTA693350.036.32370453
TCATTAA692550.036.20184354
AAGAGCA437200.035.28796814
Produced by FastQC (version 0.11.2)