FastQCFastQC Report
Sat 9 Sep 2017
HNJJKAFXX_n01_733.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJJKAFXX_n01_733.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6222310
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG4746417.628051318561756TruSeq Adapter, Index 7 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG89160.14329083571856754No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAG668200.057.51735769
GCTTGAA678550.056.89842660
CTTGAAA690750.056.16229261
TGAAAAA731900.054.5130163
TTGAAAA714200.054.45834462
GAAAAAA738650.054.4496564
TGCTTGA716550.053.24064659
CTGCTTG823750.046.1655158
TCGGGGG5400.044.088054
TCTGCTT927800.041.09973557
TTCTGCT957850.039.50090856
CTTCTGC1004000.038.32808755
CGTGTGC57550.038.197021
TCTTCTG1033950.036.8541554
GTCTTCT1035650.036.82696553
CGTCTTC1059350.036.35804752
CCGTCTT1067600.036.2257451
GCCGTCT1073000.035.97912250
TGCCGTC1124050.034.59314349
ATGCCGT1139100.034.1209548
Produced by FastQC (version 0.11.2)