FastQCFastQC Report
Sat 9 Sep 2017
HNJJKAFXX_n01_733-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJJKAFXX_n01_733-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7251156
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG4940856.813879055973972TruSeq Adapter, Index 8 (100% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAG112590.1552717939043099TruSeq Adapter, Index 8 (98% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG110300.15211367677098658TruSeq Adapter, Index 8 (98% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105160.14502515185164958No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGGCTTCTGCTTGAAAAAAAAAAG72830.1004391575632906TruSeq Adapter, Index 8 (98% over 63bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAG803250.058.3526469
GCTTGAA767200.057.79676460
CTTGAAA787350.056.84780561
TGAAAAA862750.055.60641563
TTGAAAA815550.055.37705662
GAAAAAA874000.055.3682864
TGCTTGA796450.054.1403959
CTGCTTG932000.046.01080758
TCTGCTT1055750.040.9797957
GCGTGAA46200.039.95024560
TTCTGCT1067100.039.715256
CGTGTGC65900.037.9154781
CTTCTGC1170650.037.6970255
TGCGTGA47100.036.3359
TCTTCTG1192200.036.1388454
GTCTTCT1188250.036.12857453
CGTGAAA51600.036.03684261
CGTCTTC1228900.035.7419952
CCGTCTT1267850.035.12581351
GCCGTCT1257300.035.01994750
Produced by FastQC (version 0.11.2)