FastQCFastQC Report
Sat 9 Sep 2017
HNJJKAFXX_n01_666.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJJKAFXX_n01_666.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8759552
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG135480815.466635736622148TruSeq Adapter, Index 5 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG227480.2596936464330596No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG177630.20278434330888154TruSeq Adapter, Index 5 (98% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAT110160.12575985621182453TruSeq Adapter, Index 5 (100% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATG97180.11094174679253002TruSeq Adapter, Index 5 (100% over 63bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA93830.10711735029371365TruSeq Adapter, Index 5 (100% over 63bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAG1673900.063.1339569
GAAAAAA1817600.060.4996664
CTTGAAA1788050.059.9845361
TTGAAAA1803400.059.56578462
GCTTGAA1798450.059.42822360
TGAAAAA1840350.059.40462563
TGCTTGA1928800.054.85395459
CTGCTTG2270100.046.54002458
TGACTGG28050.044.8987352
GTGACTG28150.043.8867381
GGTCGGA18600.043.4087262
CGTGTGC166950.042.6010441
GACTGGA29600.042.4298443
AGGTCGG19350.042.088141
TCTGCTT2515400.042.0698157
AGACGTG30000.041.97289314
CAGACGT31200.040.69119313
TCAGACG31500.040.63245412
TTCTGCT2600000.040.50806456
GGAGTTC31900.040.1229557
Produced by FastQC (version 0.11.2)