FastQCFastQC Report
Sat 9 Sep 2017
HNJJKAFXX_n01_666-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJJKAFXX_n01_666-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7658450
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG131937017.227637446219536TruSeq Adapter, Index 6 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG169910.22185951465374848No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA117740.15373868080355685TruSeq Adapter, Index 6 (100% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAT117230.15307274970783905TruSeq Adapter, Index 6 (100% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAC114280.14922079533064783TruSeq Adapter, Index 6 (100% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATG97830.12774125312563248TruSeq Adapter, Index 6 (100% over 63bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG81830.10684929718154457TruSeq Adapter, Index 6 (98% over 63bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA76630.100059411499716TruSeq Adapter, Index 6 (100% over 63bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAG1565550.063.11870669
GAAAAAA1703050.060.53503464
CTTGAAA1698900.059.92949361
TGAAAAA1724600.059.50454363
TTGAAAA1713300.059.5043362
GCTTGAA1713050.059.31363360
TGCTTGA1840800.054.9641959
CTGCTTG2106750.047.9939358
TCTGCTT2315750.043.68688257
TCGGGGG8550.043.183594
CGTGTGC120550.043.087351
TTCTGCT2413150.041.95679556
GTGACTG20700.041.3486981
GGTCGGA15650.041.0117382
CTTCTGC2490550.040.86257655
AGACGTG20600.040.86165214
AGGTCGG16050.040.6412731
TCTTCTG2555700.039.7939154
GACTGGA21900.039.547793
GTCTTCT2582050.039.41987653
Produced by FastQC (version 0.11.2)