FastQCFastQC Report
Sat 9 Sep 2017
HNJJKAFXX_n01_395-lg.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJJKAFXX_n01_395-lg.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11829233
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG2297871.9425350739139216RNA PCR Primer, Index 23 (100% over 43bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTGA387650.047.9158259
GCTTGAA402050.046.6899460
CTGCTTG406450.045.64820558
CCGTCTT432500.043.7754551
GCCGTCT433600.043.58483550
CGTGTGC140200.043.2016831
CGTCTTC453850.041.6705652
CTTCTGC457800.041.13786755
TCTGCTT457000.040.77959457
CTTGAAA471100.040.0662161
GTCTTCT474650.039.59026353
TTCTGCT476350.038.9775656
TGCCGTC497600.038.0006749
TCTTCTG500350.037.5774354
GTGTGCT161900.037.3412672
ATGCCGT517600.036.61231248
AAAAAAG540550.034.4843469
ACGTGTG54600.034.3856961
TGAAAAA571650.033.9482263
TTGAAAA560650.033.93738662
Produced by FastQC (version 0.11.2)