FastQCFastQC Report
Sat 9 Sep 2017
HNJJKAFXX_n01_395-lg-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJJKAFXX_n01_395-lg-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5276746
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG5241479.933148193981669TruSeq Adapter, Index 4 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG69660.13201317630221351No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG65750.12460330665906602TruSeq Adapter, Index 4 (100% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAC63080.11954337009967884TruSeq Adapter, Index 4 (100% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG59000.1118113322111771TruSeq Adapter, Index 4 (98% over 63bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG56420.10692195531109513TruSeq Adapter, Index 4 (98% over 63bp)
GCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC54250.10280957241451455TruSeq Adapter, Index 4 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAG686050.057.86118369
GCTTGAA715700.056.66493660
TGCTTGA721850.056.042559
CTTGAAA732700.055.44437461
GAAAAAA749800.054.7964864
TTGAAAA745700.054.57547862
TGAAAAA750300.054.4840763
CGTGTGC93200.048.7320941
CTGCTTG857450.047.15963458
TCTGCTT884600.045.77721457
CTTCTGC893250.045.584155
TTCTGCT889750.045.55834656
ACGTGTG30000.045.5563621
GTCTTCT906350.044.91425353
CGTCTTC908900.044.84517752
TCTTCTG911150.044.6284254
CCGTCTT915500.044.47665851
GCCGTCT931200.043.76012850
GTGTGCT110700.040.865432
TGCCGTC1015100.040.1776349
Produced by FastQC (version 0.11.2)