FastQCFastQC Report
Sat 9 Sep 2017
HNJJKAFXX_n01_395-hg.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJJKAFXX_n01_395-hg.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16296796
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG1933481.1864172564963076TruSeq Adapter, Index 16 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT354350.046.26444650
TGCTTGA353650.045.8983859
CTGCTTG353050.045.8295258
CCGTCTT361850.045.33427451
GCTTGAA380750.043.13948460
TGCCGTC386300.042.5007949
ATGCCGT399350.041.18969748
CGTCTTC406950.040.50498252
CTTCTGC421350.038.850555
TCTGCTT419300.038.6537457
TATGCCG429550.038.20546347
GTCTTCT430200.038.0993953
AAAAAAG423650.037.9319369
CGTGTGC78650.037.915171
TTCTGCT433550.037.43089356
CATCTCG429750.036.71929640
GTATGCC449800.036.52391446
GAAAAAA481750.035.04754364
TCTTCTG472600.034.72504454
GTCCATC459200.034.5372737
Produced by FastQC (version 0.11.2)