FastQCFastQC Report
Sat 9 Sep 2017
HNJJKAFXX_n01_395-hg-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJJKAFXX_n01_395-hg-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7626741
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG1902062.4939354830588845TruSeq Adapter, Index 2 (100% over 63bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTGA301100.050.7686759
GCTTGAA304600.050.74062360
GCCGTCT307350.050.44376850
CTGCTTG302250.050.3361458
CCGTCTT315250.049.36571551
CGTCTTC333950.046.5397352
TGCCGTC335200.046.4176449
CTTCTGC333850.046.27374655
CTTGAAA338200.046.10759761
AAAAAAG339200.045.6162969
TCTGCTT336350.045.45799657
ATGCCGT344100.045.33708248
TTCTGCT342250.044.49262256
GTCTTCT346150.044.4304353
TCTTCTG357350.043.07623754
TATGCCG370550.042.0822847
GAAAAAA381400.042.00648564
TGAAAAA385650.041.2930863
GTATGCC384200.040.7577846
TTGAAAA389100.040.0849162
Produced by FastQC (version 0.11.2)