FastQCFastQC Report
Sat 9 Sep 2017
HNJJKAFXX_n01_395-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJJKAFXX_n01_395-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8555263
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG3603764.21233105282678TruSeq Adapter, Index 22 (98% over 63bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTGA524600.055.45109659
GCTTGAA531850.054.81941260
CTGCTTG534900.054.3452758
CCGTCTT551950.053.05187251
GCCGTCT560550.052.2748850
CTTCTGC571700.051.18353355
CGTCTTC573000.051.1580252
TCTGCTT570150.051.0449457
TTCTGCT580800.050.20874856
GTCTTCT584000.050.18822553
AAAAAAG566650.049.99704769
CTTGAAA588000.049.7664261
TCTTCTG601450.048.68621454
TGCCGTC606200.048.40118849
ATGCCGT623100.047.08842548
TGAAAAA633500.046.53512263
TTGAAAA630300.046.5250762
GAAAAAA641000.046.2166964
CGTGTGC103300.044.034751
TATGCCG688600.042.61411747
Produced by FastQC (version 0.11.2)